Issue 1, 2013

A multi-omic systems approach to elucidating Yersinia virulence mechanisms

Abstract

The underlying mechanisms that lead to dramatic differences between closely related pathogens are not always readily apparent. For example, the genomes of Yersinia pestis (YP) the causative agent of plague with a high mortality rate and Yersinia pseudotuberculosis (YPT) an enteric pathogen with a modest mortality rate are highly similar with some species specific differences; however the molecular causes of their distinct clinical outcomes remain poorly understood. In this study, a temporal multi-omic analysis of YP and YPT at physiologically relevant temperatures was performed to gain insights into how an acute and highly lethal bacterial pathogen, YP, differs from its less virulent progenitor, YPT. This analysis revealed higher gene and protein expression levels of conserved major virulence factors in YP relative to YPT, including the Yop virulon and the pH6 antigen. This suggests that adaptation in the regulatory architecture, in addition to the presence of unique genetic material, may contribute to the increased pathogenecity of YP relative to YPT. Additionally, global transcriptome and proteome responses of YP and YPT revealed conserved post-transcriptional control of metabolism and the translational machinery including the modulation of glutamate levels in Yersiniae. Finally, the omics data was coupled with a computational network analysis, allowing an efficient prediction of novel Yersinia virulence factors based on gene and protein expression patterns.

Graphical abstract: A multi-omic systems approach to elucidating Yersinia virulence mechanisms

Supplementary files

Article information

Article type
Paper
Submitted
18 Jul 2012
Accepted
09 Oct 2012
First published
24 Oct 2012

Mol. BioSyst., 2013,9, 44-54

A multi-omic systems approach to elucidating Yersinia virulence mechanisms

C. Ansong, A. C. Schrimpe-Rutledge, H. D. Mitchell, S. Chauhan, M. B. Jones, Y. Kim, K. McAteer, B. L. Deatherage Kaiser, J. L. Dubois, H. M. Brewer, B. C. Frank, J. E. McDermott, T. O. Metz, S. N. Peterson, R. D. Smith, V. L. Motin and J. N. Adkins, Mol. BioSyst., 2013, 9, 44 DOI: 10.1039/C2MB25287B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements