A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

Sarah L. Perry; Sudipto Guha; Ashtamurthy S. Pawate; Amrit Bhaskarla; Vinayak Agarwal; Satish K. Nair; Paul J. A. Kenis

doi:10.1039/C3LC50276G

A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

Sarah L. Perry,^a Sudipto Guha,^a Ashtamurthy S. Pawate,^a Amrit Bhaskarla,^b Vinayak Agarwal,^c Satish K. Nair^c and Paul J. A. Kenis*^a

Author affiliations

* Corresponding authors

^a Department of Chemical & Biomolecular Engineering, University of Illinois at Urbana-Champaign, USA
E-mail: kenis@illinois.edu

^b School of Molecular &Cellular Biology, University of Illinois at Urbana-Champaign, USA

^c Department of Biochemistry, University of Illinois at Urbana-Champaign, USA

Abstract

We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 Å via on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods.

$Graphical abstract: A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction$

Supplementary files

Publication details

The article was received on 26 Feb 2013, accepted on 12 Jun 2013 and first published on 13 Jun 2013

Article type: Communication

DOI: 10.1039/C3LC50276G

Citation: Lab Chip, 2013,13, 3183-3187

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A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

S. L. Perry, S. Guha, A. S. Pawate, A. Bhaskarla, V. Agarwal, S. K. Nair and P. J. A. Kenis, Lab Chip, 2013, 13, 3183
DOI: 10.1039/C3LC50276G
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Lab on a Chip

A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

Abstract

Supplementary files

Publication details

A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

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