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Issue 12, 2013
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Optoelectronic tweezers integrated with lensfree holographic microscopy for wide-field interactive cell and particle manipulation on a chip

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Abstract

We demonstrate an optoelectronic tweezer (OET) coupled to a lensfree holographic microscope for real-time interactive manipulation of cells and micro-particles over a large field-of-view (FOV). This integrated platform can record the holographic images of cells and particles over the entire active area of a CCD sensor array, perform digital image reconstruction to identify target cells, dynamically track the positions of cells and particles, and project light beams to trigger light-induced dielectrophoretic forces to pattern and sort cells on a chip. OET technology has been previously shown to be capable of performing parallel single cell manipulation over a large area. However, its throughput has been bottlenecked by the number of cells that can be imaged within the limited FOV of a conventional microscope objective lens. Integrating lensfree holographic imaging with OET solves this fundamental FOV barrier, while also creating a compact on-chip cell/particle manipulation platform. Using this unique platform, we have successfully demonstrated real-time interactive manipulation of thousands of single cells and micro-particles over an ultra-large area of e.g., 240 mm2 (i.e. 17.96 mm × 13.52 mm).

Graphical abstract: Optoelectronic tweezers integrated with lensfree holographic microscopy for wide-field interactive cell and particle manipulation on a chip

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Publication details

The article was received on 05 Feb 2013, accepted on 16 Apr 2013 and first published on 16 Apr 2013


Article type: Paper
DOI: 10.1039/C3LC50168J
Citation: Lab Chip, 2013,13, 2278-2284
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    Optoelectronic tweezers integrated with lensfree holographic microscopy for wide-field interactive cell and particle manipulation on a chip

    K. Huang, T. Su, A. Ozcan and P. Chiou, Lab Chip, 2013, 13, 2278
    DOI: 10.1039/C3LC50168J

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