Issue 10, 2013
From the journal:

Lab on a Chip

Optically clear alginate hydrogels for spatially controlled cell entrapment and culture at microfluidic electrode surfaces†

Jordan F. Betz,ab   Yi Cheng,bc   Chen-Yu Tsao,d   Amin Zargar,ad   Hsuan-Chen Wu,d   Xiaolong Luo,d   Gregory F. Payne,ad   William E. Bentleyad  and  Gary W. Rubloff*bc  

* Corresponding authors

a Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA

b Institute for Systems Research, University of Maryland, College Park, MD, USA
E-mail: rubloff@umd.edu
Fax: +01 301-314-9920
Tel: +01 301-405-3011

c Department of Materials Science and Engineering, University of Maryland, College Park, MD, USA

d Institute for Bioscience & Biotechnology Research, University of Maryland, College Park, MD, USA

Abstract

We describe an innovation in the immobilization, culture, and imaging of cells in calcium alginate within microfluidic devices. This technique allows unprecedented optical access to the entirety of the calcium alginate hydrogel, enabling observation of growth and behavior in a chemical and mechanical environment favored by many kinds of cells.

Graphical abstract: Optically clear alginate hydrogels for spatially controlled cell entrapment and culture at microfluidic electrode surfaces

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Article information

DOI
https://doi.org/10.1039/C3LC50079A
Article type
Technical Innovation
Submitted
16 Jan 2013
Accepted
18 Mar 2013
First published
05 Apr 2013

Lab Chip, 2013,13, 1854-1858

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Optically clear alginate hydrogels for spatially controlled cell entrapment and culture at microfluidic electrode surfaces

J. F. Betz, Y. Cheng, C. Tsao, A. Zargar, H. Wu, X. Luo, G. F. Payne, W. E. Bentley and G. W. Rubloff, Lab Chip, 2013, 13, 1854 DOI: 10.1039/C3LC50079A

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