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Issue 1, 2013
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Full range physiological mass transport control in 3D tissue cultures

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Abstract

We report the first demonstration of a microfluidic platform that captures the full physiological range of mass transport in 3-D tissue culture. The basis of our method used long microfluidic channels connected to both sides of a central microtissue chamber at different downstream positions to control the mass transport distribution within the chamber. Precise control of the Péclet number (Pe), defined as the ratio of convective to diffusive transport, over nearly five orders of magnitude (0.0056 to 160) was achieved. The platform was used to systematically investigate the role of physiological mass transport on vasculogenesis. We demonstrate, for the first time, that vasculogenesis can be independently stimulated by interstitial flow (Pe > 10) or hypoxic conditions (Pe < 0.1), and not by the intermediate state (normal living tissue). This simple platform can be applied to physiological and biological studies of 3D living tissue followed by pathological disease studies, such as cancer research and drug screening.

Graphical abstract: Full range physiological mass transport control in 3D tissue cultures

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Publication details

The article was received on 11 Jul 2012, accepted on 09 Oct 2012 and first published on 22 Oct 2012


Article type: Paper
DOI: 10.1039/C2LC40787F
Citation: Lab Chip, 2013,13, 81-89
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    Full range physiological mass transport control in 3D tissue cultures

    Y. Hsu, M. L. Moya, P. Abiri, C. C.W. Hughes, S. C. George and A. P. Lee, Lab Chip, 2013, 13, 81
    DOI: 10.1039/C2LC40787F

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