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Issue 87, 2013
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Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry

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Abstract

We describe a general methodology for fluorescent labelling of peptide conjugates of phosphorodiamidate morpholino oligonucleotides (PMOs) by alkyne functionalization of peptides, subsequent conjugation to PMOs and labelling with a fluorescent compound (Cy5-azide). Two peptide–PMO (PPMO) examples are shown. No detrimental effect of such labelled PMOs was seen in a biological assay.

Graphical abstract: Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry

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Publication details

The article was received on 08 Aug 2013, accepted on 12 Sep 2013 and first published on 13 Sep 2013


Article type: Communication
DOI: 10.1039/C3CC46067C
Citation: Chem. Commun., 2013,49, 10260-10262
  • Open access: Creative Commons BY license
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    Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry

    F. Shabanpoor and M. J. Gait, Chem. Commun., 2013, 49, 10260
    DOI: 10.1039/C3CC46067C

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