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Issue 84, 2013
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Sandwich assay for mixed-sequence recognition of double-stranded DNA: Invader-based detection of targets specific to foodborne pathogens

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Abstract

A 96-well plate sandwich assay based on Invader capture/signalling probes is used to recognize 28-mer mixed-sequence dsDNA targets specific to Salmonella enterica, Campylobacter jejuni, Escherichia coli. Targets are detected down to 20–55 pM concentration with excellent binding specificity.

Graphical abstract: Sandwich assay for mixed-sequence recognition of double-stranded DNA: Invader-based detection of targets specific to foodborne pathogens

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Publication details

The article was received on 26 Jul 2013, accepted on 09 Sep 2013 and first published on 09 Sep 2013


Article type: Communication
DOI: 10.1039/C3CC45705B
Citation: Chem. Commun., 2013,49, 9851-9853
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    Sandwich assay for mixed-sequence recognition of double-stranded DNA: Invader-based detection of targets specific to foodborne pathogens

    B. Denn, S. Karmakar, D. C. Guenther and P. J. Hrdlicka, Chem. Commun., 2013, 49, 9851
    DOI: 10.1039/C3CC45705B

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