Jump to main content
Jump to site search

Issue 88, 2013
Previous Article Next Article

A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

Author affiliations

Abstract

The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.

Graphical abstract: A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

Back to tab navigation

Supplementary files

Publication details

The article was received on 26 Jun 2013, accepted on 03 Sep 2013 and first published on 09 Sep 2013


Article type: Communication
DOI: 10.1039/C3CC44783A
Citation: Chem. Commun., 2013,49, 10379-10381
  •   Request permissions

    A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

    A. P. Acharya, P. M. Nafisi, A. Gardner, J. L. MacKay, K. Kundu, S. Kumar and N. Murthy, Chem. Commun., 2013, 49, 10379
    DOI: 10.1039/C3CC44783A

Search articles by author

Spotlight

Advertisements