Issue 37, 2013
From the journal:

Chemical Communications

A label-free supersandwich electrogenerated chemiluminescence method for the detection of DNA methylation and assay of the methyltransferase activity†

Yan Li,*a   Xiaoe Luo,a   Zhao Yan,a   Jianbin Zheng*a  and  Honglan Qib  

* Corresponding authors

a Key Laboratory of Electroanalytical Chemistry, Institute of Analytical Science, Northwest University, Xi'an, Shaanxi 710069, P. R. China
E-mail: yanli@nwu.edu.cn

b Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, P. R. China

Abstract

A method based on electrogenerated chemiluminescence (ECL) for detection of DNA methylation and assay of the methyltransferase activity is developed, and it is demonstrated that the label-free ECL method is capable of detecting methyltransferase with a detection limit of 3 × 10−6 U mL−1, using a supersandwich amplification technique.

Graphical abstract: A label-free supersandwich electrogenerated chemiluminescence method for the detection of DNA methylation and assay of the methyltransferase activity

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Article information

DOI
https://doi.org/10.1039/C3CC00203A
Article type
Communication
Submitted
09 Jan 2013
Accepted
20 Mar 2013
First published
20 Mar 2013

Chem. Commun., 2013,49, 3869-3871

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A label-free supersandwich electrogenerated chemiluminescence method for the detection of DNA methylation and assay of the methyltransferase activity

Y. Li, X. Luo, Z. Yan, J. Zheng and H. Qi, Chem. Commun., 2013, 49, 3869 DOI: 10.1039/C3CC00203A

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