Jump to main content
Jump to site search

Issue 7, 2013
Previous Article Next Article

Micro-patterning of streptavidin–biotin-ampicillin/heparin on poly(tetrafluoroethylene) (PTFE) surfaces: simultaneous antimicrobial and anticoagulant activity

Author affiliations

Abstract

Formation of heterogeneous and controllable surface patterns on polymeric materials containing antimicrobial and anticoagulant components represent an attractive way of maintaining synthetic materials “clean” from adverse bio-activities. The primary surface “contaminants” are microbial films as well as blood coagulation, both affecting not only performance of internal or external devices, but often exhibiting detrimental effects on patients. In an effort to simultaneously inhibit formation of microbial films and surface blood coagulation multifunctional assemblies containing streptavidin (STR)–biotin bioconjugates were developed on poly(tetrafluoroethylene) (PTFE) surfaces. Using STR conjugated to a biotin-functionalized PTFE surface, spatially controlled micro-patterning was produced by grafting biotinylated polyethylene glycol (B-PEG) to COOH modified PTFE (MA-PTFE), followed by inkjet micro-printing of biotinylated ampicillin (B-AM) and biotinylated heparin (B-HP) molecules. These surfaces exhibit simultaneous antimicrobial and anticoagulant attributes manifested by “zone of inhibition” and anticoagulant measurements. Quantitative spectroscopic analysis revealed that the required surface density of COOH groups on PTFE is 2.94 × 10−7 g cm−2, and B-PEG and STR densities of 9.2 × 10−8 g cm−2 and 3.5 × 10−8 g cm−2, respectively, are sufficient to achieve simultaneous antimicrobial and anticoagulant responses. These studies also showed that the force required to remove STR–biotin conjugates attached to PTFE surfaces measured by atomic force microscopy is approximately 1090 pN, thus providing desirable surface mechanical stability.

Graphical abstract: Micro-patterning of streptavidin–biotin-ampicillin/heparin on poly(tetrafluoroethylene) (PTFE) surfaces: simultaneous antimicrobial and anticoagulant activity

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 12 Dec 2012, accepted on 07 Mar 2013, published on 02 Apr 2013 and first published online on 02 Apr 2013


Article type: Paper
DOI: 10.1039/C3BM00187C
Citation: Biomater. Sci., 2013,1, 711-718
  •   Request permissions

    Micro-patterning of streptavidin–biotin-ampicillin/heparin on poly(tetrafluoroethylene) (PTFE) surfaces: simultaneous antimicrobial and anticoagulant activity

    N. Aumsuwan, H. A. Pearson and M. W. Urban, Biomater. Sci., 2013, 1, 711
    DOI: 10.1039/C3BM00187C

Search articles by author