Issue 17, 2013

Application of free radical diphenylpicrylhydrazyl (DPPH) to estimate the antioxidant capacity of food samples

Abstract

The 2,2-diphenylpicrylhydrazyl (DPPH) assay is widely used in plant biochemistry to evaluate the properties of plant constituents for scavenging free radicals. The method is based on the spectrophotometric measurement of the DPPH concentration change resulting from the reaction with an antioxidant. Several protocols have been followed for this assay using different conditions such as different reaction times, solvents, pH and different compounds used as antioxidant standards. This review shows to what extent the mentioned parameters have the influence on the presented results.

Graphical abstract: Application of free radical diphenylpicrylhydrazyl (DPPH) to estimate the antioxidant capacity of food samples

Article information

Article type
Minireview
Submitted
07 Mar 2013
Accepted
11 Jun 2013
First published
11 Jun 2013

Anal. Methods, 2013,5, 4288-4295

Application of free radical diphenylpicrylhydrazyl (DPPH) to estimate the antioxidant capacity of food samples

K. Pyrzynska and A. Pękal, Anal. Methods, 2013, 5, 4288 DOI: 10.1039/C3AY40367J

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