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Issue 22, 2013
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Differentiation of microbial species and strains in coculture biofilms by multivariate analysis of laser desorption postionization mass spectra

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Abstract

7.87 to 10.5 eV vacuum ultraviolet (VUV) photon energies were used in laser desorption postionization mass spectrometry (LDPI-MS) to analyze biofilms comprised of binary cultures of interacting microorganisms. The effect of photon energy was examined using both tunable synchrotron and laser sources of VUV radiation. Principal components analysis (PCA) was applied to the MS data to differentiate species in Escherichia coliSaccharomyces cerevisiae coculture biofilms. PCA of LDPI-MS also differentiated individual E. coli strains in a biofilm comprised of two interacting gene deletion strains, even though these strains differed from the wild type K-12 strain by no more than four gene deletions each out of approximately 2000 genes. PCA treatment of 7.87 eV LDPI-MS data separated the E. coli strains into three distinct groups, two “pure” groups, and a mixed region. Furthermore, the “pure” regions of the E. coli cocultures showed greater variance by PCA at 7.87 eV photon energies compared to 10.5 eV radiation. This is consistent with the expectation that the 7.87 eV photoionization selects a subset of low ionization energy analytes while 10.5 eV is more inclusive, detecting a wider range of analytes. These two VUV photon energies therefore give different spreads via PCA and their respective use in LDPI-MS constitute an additional experimental parameter to differentiate strains and species.

Graphical abstract: Differentiation of microbial species and strains in coculture biofilms by multivariate analysis of laser desorption postionization mass spectra

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Publication details

The article was received on 21 Jul 2013, accepted on 17 Sep 2013 and first published on 17 Sep 2013


Article type: Paper
DOI: 10.1039/C3AN01389H
Citation: Analyst, 2013,138, 6844-6851
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    Differentiation of microbial species and strains in coculture biofilms by multivariate analysis of laser desorption postionization mass spectra

    C. Bhardwaj, Y. Cui, T. Hofstetter, S. Y. Liu, H. C. Bernstein, R. P. Carlson, M. Ahmed and L. Hanley, Analyst, 2013, 138, 6844
    DOI: 10.1039/C3AN01389H

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