Jump to main content
Jump to site search

Issue 32, 2013
Previous Article Next Article

Surface properties of nanostructured bio-active interfaces: impacts of surface stiffness and topography on cell–surface interactions

Author affiliations

Abstract

Due to their ability to confer key functions of the native extracellular matrix (ECM), poly(ethylene glycol) (PEG)-based and PEG-modified materials have been extensively used as biocompatible and bio-functionalized substrate systems to study the influence of environmental parameters on cell adhesion in vitro. Given wide-ranging recent evidence that ECM compliance influences a variety of cell functions, detailed determination and characterization of the specific PEG surface characteristics including topography, stiffness and chemistry is required. Here, we studied two frequently used bio-active interfaces—PEG-based and PEG-modified surfaces—to elucidate the differences between the physical surface properties, which cells can sense and respond to. For this purpose, two sets of surfaces were synthesized: the first set consisted of nanopatterned glass surfaces containing cRGD-functionalized gold nanoparticles surrounded by a passivated PEG-silane layer and the second set consisted of PEG-diacrylate (PEG-DA) hydrogels decorated with cRGD-functionalized gold nanoparticles. Although the two sets of nanostructured materials compared here were highly similar in terms of density and geometrical distribution of the presented bio-ligands, as well as in terms of mechanical bulk properties, the topography and mechanical properties of the surfaces were found to be substantially different and are described in detail. In comparison to the very stiff and ultra-smooth surface properties of the PEG-passivated glasses, the mechanical properties of PEG-DA surfaces in the biologically relevant stiffness range, together with the increased surface roughness at micro- and nanoscale levels have the potential to affect cell behavior. This potential was verified by studying the adhesive behavior of hematopoietic KG-1a and rat embryonic fibroblast (REF52) cells on both surfaces.

Graphical abstract: Surface properties of nanostructured bio-active interfaces: impacts of surface stiffness and topography on cell–surface interactions

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 14 Feb 2013, accepted on 04 Jun 2013 and first published on 21 Jun 2013


Article type: Paper
DOI: 10.1039/C3RA41579A
Citation: RSC Adv., 2013,3, 13293-13303
  •   Request permissions

    Surface properties of nanostructured bio-active interfaces: impacts of surface stiffness and topography on cell–surface interactions

    I. Platzman, C. A. Muth, C. Lee-Thedieck, D. Pallarola, R. Atanasova, I. Louban, E. Altrock and J. P. Spatz, RSC Adv., 2013, 3, 13293
    DOI: 10.1039/C3RA41579A

Search articles by author