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Issue 15, 2013
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Reversible protein affinity-labelling using bromomaleimide-based reagents

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Abstract

Reversible protein biotinylation is readily affected via conjugation with a bromomaleimide-based reagent followed by reductive cleavage. The intermediate biotinylated protein constructs are stable at physiological temperature and pH 8.0. Quantitative reversibility is elegantly delivered under mild conditions of using a stoichiometric amount of a bis-thiol, thus providing an approach that will be of general interest in chemical biology and proteomics.

Graphical abstract: Reversible protein affinity-labelling using bromomaleimide-based reagents

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Publication details

The article was received on 02 Feb 2013, accepted on 26 Feb 2013 and first published on 26 Feb 2013


Article type: Communication
DOI: 10.1039/C3OB40239H
Citation: Org. Biomol. Chem., 2013,11, 2408-2411
  • Open access: Creative Commons BY license
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    Reversible protein affinity-labelling using bromomaleimide-based reagents

    R. I. Nathani, V. Chudasama, C. P. Ryan, P. R. Moody, R. E. Morgan, R. J. Fitzmaurice, M. E. B. Smith, J. R. Baker and S. Caddick, Org. Biomol. Chem., 2013, 11, 2408
    DOI: 10.1039/C3OB40239H

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