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Issue 12, 2013
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Hetero-oligonucleotide nanoscale tiles capable of two-dimensional lattice formation as testbeds for a rapid, affordable purification methodology

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Abstract

New nanoscale hetero-oligonucleotide tiles are assembled from DNA, RNA and morpholino oligos and purified using size exclusion filtration. Homo-oligonucleotide tiles assembled from RP-cartridge processed DNA oligos are purified by nondenaturing gel electrophoresis. These tiles' purity and homogeneity are demonstrated by gel electrophoresis and their incorporation into two-dimensional arrays visualized by AFM. This purification methodology increases throughput and decreases costs for researchers who wish to screen multiple tiles for utilization in structural or analytical studies.

Graphical abstract: Hetero-oligonucleotide nanoscale tiles capable of two-dimensional lattice formation as testbeds for a rapid, affordable purification methodology

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Publication details

The article was received on 28 Mar 2013, accepted on 28 Apr 2013 and first published on 07 May 2013


Article type: Communication
DOI: 10.1039/C3NR01551C
Citation: Nanoscale, 2013,5, 5266-5268
  • Open access: Creative Commons BY license
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    Hetero-oligonucleotide nanoscale tiles capable of two-dimensional lattice formation as testbeds for a rapid, affordable purification methodology

    P. S. Lukeman, Nanoscale, 2013, 5, 5266
    DOI: 10.1039/C3NR01551C

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