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Issue 9, 2013
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Iron distribution through the developmental stages of Medicago truncatula nodules

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Abstract

Paramount to symbiotic nitrogen fixation (SNF) is the synthesis of a number of metalloenzymes that use iron as a critical component of their catalytical core. Since this process is carried out by endosymbiotic rhizobia living in legume root nodules, the mechanisms involved in iron delivery to the rhizobia-containing cells are critical for SNF. In order to gain insight into iron transport to the nodule, we have used synchrotron-based X-ray fluorescence to determine the spatio-temporal distribution of this metal in nodules of the legume Medicago truncatula with hitherto unattained sensitivity and resolution. The data support a model in which iron is released from the vasculature into the apoplast of the infection/differentiation zone of the nodule (zone II). The infected cell subsequently takes up this apoplastic iron and delivers it to the symbiosome and the secretory system to synthesize ferroproteins. Upon senescence, iron is relocated to the vasculature to be reused by the shoot. These observations highlight the important role of yet to be discovered metal transporters in iron compartmentalization in the nodule and in the recovery of an essential and scarce nutrient for flowering and seed production.

Graphical abstract: Iron distribution through the developmental stages of Medicago truncatula nodules

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Publication details

The article was received on 28 Feb 2013, accepted on 05 Apr 2013 and first published on 05 Apr 2013


Article type: Paper
DOI: 10.1039/C3MT00060E
Citation: Metallomics, 2013,5, 1247-1253
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    Iron distribution through the developmental stages of Medicago truncatula nodules

    B. Rodríguez-Haas, L. Finney, S. Vogt, P. González-Melendi, J. Imperial and M. González-Guerrero, Metallomics, 2013, 5, 1247
    DOI: 10.1039/C3MT00060E

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