Biosensing MAPs as “roadblocks”: kinesin-based functional analysis of tau protein isoforms and mutants using suspended microtubules (sMTs)†
Abstract
The concept of a reconstructed microtubule kinesin-based transport system was originally introduced for studies of underlying biophysical mechanisms of intracellular transport and its potential applications in bioengineering at micro- and nanoscale levels. However, several technically challenging shortcomings prohibit its use in practical applications. One of them is the propensity of microtubules to bind various