Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Volume 161, 2013
Previous Article Next Article

Transient pearling and vesiculation of membrane tubes under osmotic gradients

Author affiliations

Abstract

We report the experimental observation of osmotically induced transient pearling instabilities in vesicular membranes. Giant phospholipid vesicles subjected to negative osmotic gradient, which drives the influx of water in to the vesicular interior, produces transient cylindrical protrusions. These protrusions exhibit a remarkable pearling intermediate, which facilitates their subsequent retraction. The pearling front propagates from the distal free end of the protrusion toward the vesicular source and accompanies gradual shortening of the protrusion via pearl–pearl coalescence. Real-time introduction of a positive osmotic gradient, on the other hand, drives vigorous shape fluctuations, which in turn produce cylindrical, prolate- and pear-shaped intermediates presumably due to an increased vesicular area relative to the encapsulated volume. These intermediates transiently produce a pearled state prior to their fission. In both cases, the transient pearling state gives rise to an array of stable spherical daughter vesicles, which may be connected to one another by fine tethers not resolved in our experiments. These results may have implications for self-reproduction in primitive, protein-free, cells.

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 09 Jun 2012, accepted on 03 Jul 2012 and first published on 03 Jul 2012


Article type: Paper
DOI: 10.1039/C2FD20116J
Citation: Faraday Discuss., 2013,161, 167-176
  •   Request permissions

    Transient pearling and vesiculation of membrane tubes under osmotic gradients

    J. Sanborn, K. Oglęcka, R. S. Kraut and A. N. Parikh, Faraday Discuss., 2013, 161, 167
    DOI: 10.1039/C2FD20116J

Search articles by author