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Issue 1, 2013
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Palladium-mediated bioorthogonal conjugation of dual-functionalised nanoparticles and their cellular delivery

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Abstract

Nanoparticles have gained considerable significance in the life sciences due to their ability to be internalised by living cells and the relative ease with which they can be functionalised with cargos ranging from molecular sensors to biomacromolecules. However, the scope of available bioconjugation methods is limited and new bioorthogonal methods are much sought after. Herein, we present dual functionalised (HO)2B/H2N-polymeric nanoparticles which can be conjugated via amide bond formation and/or Pd-mediated Suzuki–Miyaura cross-coupling in a chemoselective and bioorthogonal manner. These dual-functionalised particles were found to be efficiently taken up by mammalian cells without toxicity and were successfully employed in the cellular delivery and intracellular release of a “turn-on” molecular probe thereby demonstrating the potential use of the new particles for chemical biology applications.

Graphical abstract: Palladium-mediated bioorthogonal conjugation of dual-functionalised nanoparticles and their cellular delivery

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Publication details

The article was received on 02 Jun 2012, accepted on 09 Oct 2012 and first published on 11 Oct 2012


Article type: Edge Article
DOI: 10.1039/C2SC20706K
Citation: Chem. Sci., 2013,4, 425-431
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    Palladium-mediated bioorthogonal conjugation of dual-functionalised nanoparticles and their cellular delivery

    F. Thielbeer, S. V. Chankeshwara, E. M. V. Johansson, N. Norouzi and M. Bradley, Chem. Sci., 2013, 4, 425
    DOI: 10.1039/C2SC20706K

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