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Issue 9, 2012
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Genes for iron metabolism influence circadian rhythms in Drosophila melanogaster

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Abstract

Haem has been previously implicated in the function of the circadian clock, but whether iron homeostasis is integrated with circadian rhythms is unknown. Here we describe an RNA interference (RNAi) screen using clock neurons of Drosophila melanogaster. RNAi is targeted to iron metabolism genes, including those involved in haem biosynthesis and degradation. The results indicate that Ferritin 2 Light Chain Homologue (Fer2LCH) is required for the circadian activity of flies kept in constant darkness. Oscillations of the core components in the molecular clock, PER and TIM, were also disrupted following Fer2LCH silencing. Other genes with a putative function in circadian biology include Transferrin-3, CG1358 (which has homology to the FLVCR haem export protein) and five genes implicated in iron–sulfur cluster biosynthesis: the Drosophila homologues of IscS (CG12264), IscU (CG9836), IscA1 (CG8198), Iba57 (CG8043) and Nubp2 (CG4858). Therefore, Drosophila genes involved in iron metabolism are required for a functional biological clock.

Graphical abstract: Genes for iron metabolism influence circadian rhythms in Drosophila melanogaster

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Publication details

The article was received on 06 Apr 2012, accepted on 25 Jul 2012 and first published on 25 Jul 2012


Article type: Paper
DOI: 10.1039/C2MT20065A
Citation: Metallomics, 2012,4, 928-936
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    Genes for iron metabolism influence circadian rhythms in Drosophila melanogaster

    K. Mandilaras and F. Missirlis, Metallomics, 2012, 4, 928
    DOI: 10.1039/C2MT20065A

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