Issue 9, 2012
From the journal:

Molecular BioSystems

Porphyrins from a metagenomic library of the marine sponge Discodermia calyx†

Rui He,ac   Toshiyuki Wakimoto,*a   Yuya Takeshige,a   Yoko Egami,a   Hiromichi Kenmoku,b   Takuya Ito,b   Bochu Wang,*c   Yoshinori Asakawab  and  Ikuro Abe*a  

* Corresponding authors

a Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
E-mail: abei@mol.f.u-tokyo.ac.jp
Fax: +81 3 5841 4744
Tel: +81 3 5841 4741

b Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan
E-mail: asakawa@ph.bunri-u.ac.jp
Fax: 81 88 655 3051
Tel: 81 88 622 9611

c Bioengineering College, Chongqing University, No. 174, Shanpingba Main Street, Chongqing, P. R. China
E-mail: wangbc2000@126.com
Fax: +86 23 65112877
Tel: +86 23 65112840

Abstract

Marine sponges harbouring uncultured symbiotic bacteria are important sources of biologically active compounds. Since they would be interesting resources to explore unknown functional genes by means of a metagenomic approach, we constructed a metagenomic library of the Japanese marine sponge Discodermia calyx. The functional screening afforded the two clones producing porphyrins as red pigments. The isolation and structural elucidation of the red pigments revealed that the major red pigment was Zn-coproporphyrin III. The sequence data of the clones identified genes encoding glutamyl-tRNA reductase along with other ORFs related to porphyrin biosynthesis.

Graphical abstract: Porphyrins from a metagenomic library of the marine sponge Discodermia calyx

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Article information

DOI
https://doi.org/10.1039/C2MB25169H
Article type
Paper
Submitted
11 Apr 2012
Accepted
22 May 2012
First published
24 May 2012

Mol. BioSyst., 2012,8, 2334-2338

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Porphyrins from a metagenomic library of the marine sponge Discodermia calyx

R. He, T. Wakimoto, Y. Takeshige, Y. Egami, H. Kenmoku, T. Ito, B. Wang, Y. Asakawa and I. Abe, Mol. BioSyst., 2012, 8, 2334 DOI: 10.1039/C2MB25169H

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