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Issue 3, 2012
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Differential binding of 2′-biotinylated analogs of c-di-GMP with c-di-GMP riboswitches and binding proteins

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Abstract

C-di-GMP has emerged as a signalling molecule that regulates a variety of processes in several bacteria; therefore there is interest in the development of biotinylated analogs for the identification of binding partners. No detailed study has been done to evaluate if biotinylated analogs of c-di-GMP are capable of binding to c-di-GMP receptors. Herein, we evaluate the binding of commercially available 2′-biotinylated c-di-GMP and phosphorothioate 2′-biotinylated c-di-GMP, prepared via a facile solid-phase synthesis, to several c-di-GMP receptors. Docking, using Autodock vina software, as well as experimental studies of these analogs, with c-di-GMP class I and II riboswitches and binding proteins, reveal that some, but not all, c-di-GMP receptors can tolerate the 2′-modification of c-di-GMP with biotin.

Graphical abstract: Differential binding of 2′-biotinylated analogs of c-di-GMP with c-di-GMP riboswitches and binding proteins

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Publication details

The article was received on 18 Aug 2011, accepted on 21 Nov 2011 and first published on 19 Dec 2011


Article type: Paper
DOI: 10.1039/C2MB05338A
Citation: Mol. BioSyst., 2012,8, 772-778
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    Differential binding of 2′-biotinylated analogs of c-di-GMP with c-di-GMP riboswitches and binding proteins

    Y. Luo, J. Zhou, S. K. Watt, V. T. Lee, T. K. Dayie and H. O. Sintim, Mol. BioSyst., 2012, 8, 772
    DOI: 10.1039/C2MB05338A

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