Issue 23, 2012
From the journal:

Lab on a Chip

A simple packed bed device for antibody labelled rare cell capture from whole blood†

Jason G Kralj,a   Chandamany Arya,b   Alessandro Tona,a   Thomas P Forbes,a   Matthew S Munson,a   Lynn Sorbara,c   Sudhir Srivastavac  and  Samuel P Forry*a  

* Corresponding authors

a Biochemical Science Division, NIST, 100 Bureau Dr Gaithersburg, MD USA
E-mail: samuel.forry@nist.gov
Fax: 01 (301) 330-3447
Tel: 01 (301) 975-5246

b Department of Mechanical Engineering, University of Maryland, College Park, MD USA

c Early Detection Research Network, NCI, NIH Bethesda, MD USA

Abstract

We have developed a system to isolate rare cells from whole blood using commercially available components and simple microfluidics. We characterized the capture of MCF-7 cells spiked into whole human blood using this system to demonstrate that enrichment and enumeration studies give results similar to in situ surface-modified devices while reducing fabrication and operation complexity.

Graphical abstract: A simple packed bed device for antibody labelled rare cell capture from whole blood

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Article information

DOI
https://doi.org/10.1039/C2LC41048F
Article type
Technical Innovation
Submitted
07 Aug 2012
Accepted
24 Sep 2012
First published
26 Sep 2012

Lab Chip, 2012,12, 4972-4975

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A simple packed bed device for antibody labelled rare cell capture from whole blood

J. G. Kralj, C. Arya, A. Tona, T. P. Forbes, M. S. Munson, L. Sorbara, S. Srivastava and S. P. Forry, Lab Chip, 2012, 12, 4972 DOI: 10.1039/C2LC41048F

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