Jump to main content
Jump to site search

Issue 24, 2012
Previous Article Next Article

Simultaneous detection of duplex DNA oligonucleotides using a SERS-based micro-network gradient chip

Author affiliations

Abstract

We report the development of a programmable surface-enhanced Raman scattering (SERS)-based micro-network gradient platform to simultaneously detect two different types of DNA oligomer mixtures. The utility of this platform was demonstrated by quantitative analysis of two breast cancer-related (BRCA1) DNA oligomer mixtures. To generate on-demand concentration gradients, the microfluidic circuit was designed using an electric-hydraulic analogy. Then a multi-gradient microfluidic channel was fabricated based on the theoretical design of the concentration control module. These micro-network structures automatically produce a series of different concentration gradients by continuously mixing Cy3-labeled DNA oligomers (BRAC1-Mutation) with TAMRA-labeled DNA oligomer (BRAC1-Wild). The SERS signals for different ratios of duplex DNA oligomer mixtures, adsorbed on the surface of silver nanoparticles, were measured under flowing conditions. Total analysis time from serial mixing to SERS detection takes less than 10 min because all experimental conditions are automatically controlled inside the exquisitely designed microfluidic channel. This novel SERS-based DNA sensing technology in a micro-network gradient channel is expected to be a powerful analytical tool to simultaneously detect multiple DNA oligomer mixtures.

Graphical abstract: Simultaneous detection of duplex DNA oligonucleotides using a SERS-based micro-network gradient chip

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 04 Aug 2012, accepted on 25 Sep 2012 and first published on 26 Sep 2012


Article type: Paper
DOI: 10.1039/C2LC40890B
Citation: Lab Chip, 2012,12, 5160-5167
  •   Request permissions

    Simultaneous detection of duplex DNA oligonucleotides using a SERS-based micro-network gradient chip

    N. Choi, K. Lee, D. W. Lim, E. K. Lee, S. Chang, K. W. Oh and J. Choo, Lab Chip, 2012, 12, 5160
    DOI: 10.1039/C2LC40890B

Search articles by author