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Issue 21, 2012
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A novel microfluidic microplate as the next generation assay platform for enzyme linked immunoassays (ELISA)

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Abstract

In this work we introduce a novel microfluidic enzyme linked immunoassays (ELISA) microplate as the next generation assay platform for unparalleled assay performances. A combination of microfluidic technology with standard SBS-configured 96-well microplate architecture, in the form of microfluidic microplate technology, allows for the improvement of ELISA workflows, conservation of samples and reagents, improved reaction kinetics, and the ability to improve the sensitivity of the assay by multiple analyte loading. This paper presents the design and characterization of the microfluidic microplate, and its application in ELISA.

Graphical abstract: A novel microfluidic microplate as the next generation assay platform for enzyme linked immunoassays (ELISA)

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Publication details

The article was received on 21 May 2012, accepted on 23 Jul 2012 and first published on 27 Jul 2012


Article type: Paper
DOI: 10.1039/C2LC40585G
Citation: Lab Chip, 2012,12, 4257-4262
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    A novel microfluidic microplate as the next generation assay platform for enzyme linked immunoassays (ELISA)

    J. Kai, A. Puntambekar, N. Santiago, S. H. Lee, D. W. Sehy, V. Moore, J. Han and C. H. Ahn, Lab Chip, 2012, 12, 4257
    DOI: 10.1039/C2LC40585G

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