Jump to main content
Jump to site search

Issue 19, 2012
Previous Article Next Article

Optofluidic device for label-free cell classification from whole blood

Author affiliations

Abstract

We demonstrated a unique optofluidic lab-on-a-chip device that can measure optically encoded forward scattering signals. From the design of the spatial pattern, we can measure the position and velocity of each cell in the flow and generate a 2-D cell distribution plot over the cross section of the channel. Moreover, we have demonstrated that the cell distribution is highly sensitive to its size and stiffness. The latter is an important biomarker for cell classification and our method offers a simple and unequivocal method to classify cells by their size and stiffness. We have proved the concept using live and fixed HeLa cells. Due to the stiffness and size difference of neutrophils compared to other types of white blood cells, we have demonstrated detection of neutrophils from other blood cells. Finally, we have performed the test using 5 μL of human blood. In a greatly simplified blood preparation process, skipping the usual steps of anticoagulation, centrifuge, antibody labelling or staining, filtering, etc., we have demonstrated that our device and detection principle can count neutrophils in whole human blood. Our system is compact, inexpensive and simple to fabricate and operate, having a commodity laser diode and a Si PIN photoreceiver as the main pieces of hardware. Although the results are still preliminary, the studies indicate that this optofluidic device holds promise to be a point-of-care and home care device to measure neutrophil concentration, which is the key indicator of the immune functions for cancer patients undergoing chemotherapy.

Graphical abstract: Optofluidic device for label-free cell classification from whole blood

Back to tab navigation

Supplementary files

Publication details

The article was received on 16 May 2012, accepted on 16 Jul 2012 and first published on 16 Jul 2012


Article type: Paper
DOI: 10.1039/C2LC40560A
Citation: Lab Chip, 2012,12, 3791-3797
  •   Request permissions

    Optofluidic device for label-free cell classification from whole blood

    T. Wu, Z. Mei and Y. Lo, Lab Chip, 2012, 12, 3791
    DOI: 10.1039/C2LC40560A

Search articles by author

Spotlight

Advertisements