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Issue 18, 2012
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Droplet microfluidics for amplification-free genetic detection of single cells

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Abstract

In this article we present a novel droplet microfluidic chip enabling amplification-free detection of single pathogenic cells. The device streamlines multiple functionalities to carry out sample digitization, cell lysis, probe–target hybridization for subsequent fluorescent detection. A peptide nucleic acid fluorescence resonance energy transfer probe (PNA beacon) is used to detect 16S rRNA present in pathogenic cells. Initially the sensitivity and quantification abilities of the platform are tested using a synthetic target mimicking the actual expression level of 16S rRNA in single cells. The capability of the device to perform “sample-to-answer” pathogen detection of single cells is demonstrated using E. coli as a model pathogen.

Graphical abstract: Droplet microfluidics for amplification-free genetic detection of single cells

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Publication details

The article was received on 10 May 2012, accepted on 29 Jun 2012 and first published on 04 Jul 2012


Article type: Paper
DOI: 10.1039/C2LC40537G
Citation: Lab Chip, 2012,12, 3341-3347
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    Droplet microfluidics for amplification-free genetic detection of single cells

    T. D. Rane, H. C. Zec, C. Puleo, A. P. Lee and T. Wang, Lab Chip, 2012, 12, 3341
    DOI: 10.1039/C2LC40537G

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