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Issue 20, 2012
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A single-cell drug efflux assay in bacteria by using a directly accessible femtoliter droplet array

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Abstract

Active efflux of drugs, such as antibiotics, from a cell is one of the major mechanisms that cause multi-drug resistance in bacteria. Here we report a method to assess drug efflux activity in individual Escherichia coli cells enclosed and isolated in a directly accessible femtoliter droplet array with a fluorogenic compound. The inhibitory effect of a chemical compound on an exogenously expressed efflux pump system from pathogenic bacteria was easily detected at the single-cell level. We also present a proof-of-principle experiment to screen for the gene encoding a drug efflux pump by collecting individual droplets containing single cells in which the drug efflux activity was restored after introduction of the exogenous gene from pathogenic bacteria. Our approach will be a useful tool to screen novel pump inhibitors and efflux pump genes, and to overcome infectious diseases caused by multi-drug-resistant bacteria.

Graphical abstract: A single-cell drug efflux assay in bacteria by using a directly accessible femtoliter droplet array

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Publication details

The article was received on 23 Apr 2012, accepted on 07 Jun 2012 and first published on 13 Jun 2012


Article type: Paper
DOI: 10.1039/C2LC40394C
Citation: Lab Chip, 2012,12, 3923-3929
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    A single-cell drug efflux assay in bacteria by using a directly accessible femtoliter droplet array

    R. Iino, K. Hayama, H. Amezawa, S. Sakakihara, S. H. Kim, Y. Matsumono, K. Nishino, A. Yamaguchi and H. Noji, Lab Chip, 2012, 12, 3923
    DOI: 10.1039/C2LC40394C

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