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Issue 7, 2012
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Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter

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Abstract

We report a high speed and high purity pulsed laser triggered fluorescence activated cell sorter (PLACS) with a sorting throughput up to 20 000 mammalian cells s−1 with 37% sorting purity, 90% cell viability in enrichment mode, and >90% purity in high purity mode at 1500 cells s−1 or 3000 beads s−1. Fast switching (30 μs) and a small perturbation volume (∼90 pL) is achieved by a unique sorting mechanism in which explosive vapor bubbles are generated using focused laser pulses in a single layer microfluidic PDMS channel.

Graphical abstract: Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter

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Publication details

The article was received on 08 Nov 2011, accepted on 09 Feb 2012 and first published on 15 Feb 2012


Article type: Paper
DOI: 10.1039/C2LC21084C
Citation: Lab Chip, 2012,12, 1378-1383
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    Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter

    T. Wu, Y. Chen, S. Park, J. Hong, T. Teslaa, J. F. Zhong, D. Di Carlo, M. A. Teitell and P. Chiou, Lab Chip, 2012, 12, 1378
    DOI: 10.1039/C2LC21084C

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