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Issue 37, 2012
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GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment

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Abstract

Multilayered polyelectrolyte coatings comprising thiolated chitosan (TC) and glycosaminoglycans (GAGs), chondroitin sulphate and hyaluronic acid, were built using a layer-by-layer approach. The surface activity of these coatings for binding and inhibition of enzymes related to chronic inflammation, such as collagenase and myeloperoxidase, was assessed. The build-up of five bi-layers of TC/GAGs onto gold surfaces was monitored in situ by QCM-D. All experimental groups showed exponential growth of the coatings controlled by the degree of chitosan thiolation and the molecular weight of the GAGs. The degree of chitosan modification was also the key parameter influencing the enzyme activity: increasing the thiols content led to more efficient myeloperoxidase inhibition and was inversely proportional to the adsorption of collagenase. Enhanced fibroblast attachment and proliferation were observed when the multilayered polyelectrolyte constructs terminated with GAGs. The possibility to control either the activity of major wound enzymes by the thiolation degree of the coating or the cell adhesion and proliferation by proper selection of the ultimate layer makes these materials potentially useful in chronic wounds treatment and dermal tissue regeneration.

Graphical abstract: GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment

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Publication details

The article was received on 20 Feb 2012, accepted on 20 Apr 2012 and first published on 24 Apr 2012


Article type: Paper
DOI: 10.1039/C2JM31051A
Citation: J. Mater. Chem., 2012,22, 19438-19446
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    GAGs-thiolated chitosan assemblies for chronic wounds treatment: control of enzyme activity and cell attachment

    A. Francesko, D. Soares da Costa, P. Lisboa, R. L. Reis, I. Pashkuleva and T. Tzanov, J. Mater. Chem., 2012, 22, 19438
    DOI: 10.1039/C2JM31051A

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