Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 7, 2012
Previous Article Next Article

Self-assembled chitin nanofiber templates for artificial neural networks

Author affiliations

Abstract

Self-assembled chitin nanofibers were applied as a biomimetic extracellular matrix for the attachment of primary neuronsin vitro. Chitin nanofiber surfaces were deacetylated to form 4 nm and 12 nm diameter chitosan nanofibers that were coupled with poly-D-lysine (PDL) to examine combinatory effects and structurally analyzed by atomic force microscopy. The chitosan substrates were then employed for mouse cortical neuron cultures to examine their capabilities to support cell attachment, neurite coverage and survival. The 4 nm chitosan nanofibers improved single cortical neuron attachment compared to the 12 nm chitosan fibers and bare glass substrates, illustrating the improved adhesive properties of the surface. Importantly, the 4 nm chitosan nanofibers with PDL supported 37.9% neuron viability compared to only 13.5% on traditional PDL surfaces after a 7-day culture period, illustrating significantly improved long-term cell viability. The nanofibrillar chitosan surface could provide an alternative substrate for in vitroprimary neuron cultures to serve as artificial neural networks for diagnostics and therapeutics.

Graphical abstract: Self-assembled chitin nanofiber templates for artificial neural networks

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 27 Oct 2011, accepted on 05 Dec 2011 and first published on 05 Jan 2012


Article type: Paper
DOI: 10.1039/C2JM15487K
Citation: J. Mater. Chem., 2012,22, 3105-3109
  •   Request permissions

    Self-assembled chitin nanofiber templates for artificial neural networks

    A. Cooper, C. Zhong, Y. Kinoshita, R. S. Morrison, M. Rolandi and M. Zhang, J. Mater. Chem., 2012, 22, 3105
    DOI: 10.1039/C2JM15487K

Search articles by author