Jump to main content
Jump to site search

Issue 3, 2013
Previous Article Next Article

Conserved host–pathogen PPIs
Globally conserved inter-species bacterial PPIs based conserved host-pathogen interactome derived novel target in C. pseudotuberculosis, C. diphtheriae, M. tuberculosis, C. ulcerans, Y. pestis, and E. coli targeted by Piper betel compounds

Author affiliations

Abstract

Although attempts have been made to unveil proteinprotein and host–pathogen interactions based on molecular insights of important biological events and pathogenesis in various organisms, these efforts have not yet been reported in Corynebacterium pseudotuberculosis (Cp), the causative agent of Caseous Lymphadenitis (CLA). In this study, we used computational approaches to develop common conserved intra-species proteinprotein interaction (PPI) networks first time for four Cp strains (Cp FRC41, Cp 316, Cp 3/99-5, and Cp P54B96) followed by development of a common conserved inter-species bacterial PPI using conserved proteins in multiple pathogens (Y. pestis, M. tuberculosis, C. diphtheriae, C. ulcerans, E. coli, and all four Cp strains) and E. Coli based experimentally validated PPI data. Furthermore, the interacting proteins in the common conserved inter-species bacterial PPI were used to generate a conserved host–pathogen interaction (HP-PPI) network considering human, goat, sheep, bovine, and horse as hosts. The HP-PPI network was validated, and acetate kinase (Ack) was identified as a novel broad spectrum target. Ceftiofur, penicillin, and two natural compounds derived from Piper betel were predicted to inhibit Ack activity. One of these Piper betel compounds found to inhibit E. coli O157:H7 growth similar to penicillin. The target specificity of these betel compounds, their effects on other studied pathogens, and other in silico results are currently being validated and the results are promising.

Graphical abstract: Conserved host–pathogen PPIs Globally conserved inter-species bacterial PPIs based conserved host-pathogen interactome derived novel target in C. pseudotuberculosis, C. diphtheriae, M. tuberculosis, C. ulcerans, Y. pestis, and E. coli targeted by Piper betel compounds

Back to tab navigation

Supplementary files

Additions and corrections

Publication details

The article was received on 27 Aug 2012, accepted on 05 Nov 2012 and first published on 03 Jan 2013


Article type: Paper
DOI: 10.1039/C2IB20206A
Citation: Integr. Biol., 2013,5, 495-509
  •   Request permissions

    Conserved host–pathogen PPIs

    D. Barh, K. Gupta, N. Jain, G. Khatri, N. León-Sicairos, A. Canizalez-Roman, S. Tiwari, A. Verma, S. Rahangdale, S. Shah Hassan, A. Rodrigues dos Santos, A. Ali, L. Carlos Guimarães, R. Thiago Jucá Ramos, P. Devarapalli, N. Barve, M. Bakhtiar, R. Kumavath, P. Ghosh, A. Miyoshi, A. Silva, A. Kumar, A. Narayan Misra, K. Blum, J. Baumbach and V. Azevedo, Integr. Biol., 2013, 5, 495
    DOI: 10.1039/C2IB20206A

Search articles by author

Spotlight

Advertisements