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Issue 6, 2012
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Preparation, characterization, and surface immobilization of native vesicles obtained by mechanical extrusion of mammalian cells

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Abstract

Native vesicles or “reduced protocells” derived by mechanical extrusion concentrate selected plasma membrane components, while downsizing complexities of whole cells. We illustrate this technique, characterize the physical-chemical properties of these reduced configurations of whole cells, and demonstrate their surface immobilization and patternability. This simple detergent-free vesicularized membrane preparation should prove useful in fundamental studies of cellular membranes, and may provide a means to engineer therapeutic cells and enable high-throughput devices containing near-native, functional proteolipidic assemblies.

Graphical abstract: Preparation, characterization, and surface immobilization of native vesicles obtained by mechanical extrusion of mammalian cells

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Publication details

The article was received on 06 Feb 2012, accepted on 09 Apr 2012 and first published on 12 Apr 2012


Article type: Technical Innovation
DOI: 10.1039/C2IB20022H
Citation: Integr. Biol., 2012,4, 685-692
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    Preparation, characterization, and surface immobilization of native vesicles obtained by mechanical extrusion of mammalian cells

    H. Wu, A. E. Oliver, V. N. Ngassam, C. K. Yee, A. N. Parikh and Y. Yeh, Integr. Biol., 2012, 4, 685
    DOI: 10.1039/C2IB20022H

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