Jump to main content
Jump to site search

Issue 42, 2012
Previous Article Next Article

The ‘sticky business’ of cleaning gas-phase membrane proteins: a detergent oriented perspective

Author affiliations

Abstract

In recent years the properties of gas-phase detergent clusters have come under close scrutiny due in part to their participation in the analysis of intact membrane protein complexes by mass spectrometry. The detergent molecules that cover the protein complex are removed in the gas-phase by thermally agitating the ions by collision-induced dissociation. This process however, is not readily controlled and can frequently result in the disruption of protein structure. Improved methods of releasing proteins from detergent clusters are clearly required. To facilitate this the structural properties of detergent clusters along with the mechanistic details of their dissociation need to be understood. Pivotal to understanding the properties of gas-phase detergent clusters is the technique of ion mobility mass spectrometry. This technique can be used to assign polydisperse detergent clusters and provide information about their geometries and packing densities. In this article we consider the shapes of detergent clusters and show that these clusters possess geometries that are inconsistent with those in solution. We analyse the distributions of clusters in detail using tandem mass spectrometry and suggest that the mean charge of clusters formed from certain detergents is governed by electrostatic repulsion. We discuss the dissociation of detergent clusters and propose that detergent evaporation it a key process in the protection of protein complexes during high energy collisions in the gas-phase.

Graphical abstract: The ‘sticky business’ of cleaning gas-phase membrane proteins: a detergent oriented perspective

Back to tab navigation

Supplementary files

Publication details

The article was received on 23 May 2012, accepted on 03 Sep 2012 and first published on 04 Sep 2012


Article type: Perspective
DOI: 10.1039/C2CP41687E
Citation: Phys. Chem. Chem. Phys., 2012,14, 14439-14449
  •   Request permissions

    The ‘sticky business’ of cleaning gas-phase membrane proteins: a detergent oriented perspective

    A. J. Borysik and C. V. Robinson, Phys. Chem. Chem. Phys., 2012, 14, 14439
    DOI: 10.1039/C2CP41687E

Search articles by author

Spotlight

Advertisements