Issue 10, 2012
From the journal:

Physical Chemistry Chemical Physics

The development of direct multicolour fluorescence cross-correlation spectroscopy: Towards a new tool for tracking complex biomolecular events in real-time

Holly M. Wobma,†a   Megan L. Blades,a   Ekaterina Grekova,a   Dylan L. McGuire,b   Kun Chen,c   Warren C. W. Chanc  and  David T. Cramb*a  

* Corresponding authors

a Department of Chemistry, University of Calgary, 2500 University Dr NW, Calgary, AB, Canada
E-mail: dcramb@ucalgary.ca
Fax: +1 403 289 9488
Tel: +1 403 220 8138

b Department of Physics, McGill University, 845 Sherbrooke St. W. Montreal, Quebec, Canada

c Institute of Biomaterials and Biomedical Engineering, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College St., Toronto, ON, Canada

Abstract

Direct three-colour fluorescence cross-correlation spectroscopy can reveal interactions between three fluorescently labelled biomolecules, giving insight toward the complex events that constitute signal transduction pathways. Here we provide the optical and theoretical basis for this technology and demonstrate its ability to detect specific biological associations between nanoparticle-labelled DNA molecules.

Graphical abstract: The development of direct multicolour fluorescence cross-correlation spectroscopy: Towards a new tool for tracking complex biomolecular events in real-time

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Article information

DOI
https://doi.org/10.1039/C2CP23278B
Article type
Communication
Submitted
17 Oct 2011
Accepted
22 Dec 2011
First published
22 Dec 2011

Phys. Chem. Chem. Phys., 2012,14, 3290-3294

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The development of direct multicolour fluorescence cross-correlation spectroscopy: Towards a new tool for tracking complex biomolecular events in real-time

H. M. Wobma, M. L. Blades, E. Grekova, D. L. McGuire, K. Chen, W. C. W. Chan and D. T. Cramb, Phys. Chem. Chem. Phys., 2012, 14, 3290 DOI: 10.1039/C2CP23278B

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