Issue 88, 2012
From the journal:

Chemical Communications

Direct immobilization of oxyamine-modified proteins from cell lysates†

Long Yi,‡ab   Yong-Xiang Chen,‡a   Po-Chiao Lin,a   Hendrik Schröder,c   Christof. M. Niemeyer,cd   Yao-Wen Wu,b   Roger S. Goody,b   Gemma Triola*a  and  Herbert Waldmann*a  

* Corresponding authors

a Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, D-44227 Dortmund, Germany and Chemical Biology, Faculty of Chemistry, TU Dortmund University, Otto-Hahn-Strasse 6, D-44227 Dortmund, Germany
E-mail: herbert.waldmann@mpi-dortmund.mpg.de, gemma.triola@mpi-dortmund.mpg.de
Fax: +49 (231)1332499
Tel: +49 (231)1332401

b Department of Physical Biochemistry, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, D-44227 Dortmund, Germany

c Biologisch-Chemische Mikrostrukturtechnik, Faculty of Chemistry, TU Dortmund University, Otto-Hahn-Strasse 6, D-44227 Dortmund, Germany

d Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz, D-76344 Eggenstein-Leopoldshafen, Germany

Abstract

Oxyamine-modified proteins can be efficiently and selectively immobilized on ketone-coated glass slides at neutral pH in short reaction times by direct treatment and spotting of protein expression lysates without prior purification.

Graphical abstract: Direct immobilization of oxyamine-modified proteins from cell lysates

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Article information

DOI
https://doi.org/10.1039/C2CC35237K
Article type
Communication
Submitted
20 Jul 2012
Accepted
11 Sep 2012
First published
11 Sep 2012

Chem. Commun., 2012,48, 10829-10831

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Direct immobilization of oxyamine-modified proteins from cell lysates

L. Yi, Y. Chen, P. Lin, H. Schröder, Christof. M. Niemeyer, Y. Wu, R. S. Goody, G. Triola and H. Waldmann, Chem. Commun., 2012, 48, 10829 DOI: 10.1039/C2CC35237K

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