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Issue 79, 2012
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Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2′-modified nucleotides

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Abstract

We present a screening assay based on fluorescence readout for the directed evolution of T7 RNA polymerase variants with acceptance of 2′-modified nucleotides. By using this screening we were able to identify a T7 RNA polymerase mutant with increased acceptance of 2′-methylseleno-2′-deoxyuridine 5′-triphosphate.

Graphical abstract: Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2′-modified nucleotides

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Publication details

The article was received on 13 Jul 2012, accepted on 13 Aug 2012 and first published on 16 Aug 2012


Article type: Communication
DOI: 10.1039/C2CC35028A
Citation: Chem. Commun., 2012,48, 9870-9872
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    Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2′-modified nucleotides

    V. Siegmund, T. Santner, R. Micura and A. Marx, Chem. Commun., 2012, 48, 9870
    DOI: 10.1039/C2CC35028A

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