Issue 72, 2012
From the journal:

Chemical Communications

Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy†

Shizuka Nakayama,a   Yiling Luo,a   Jie Zhou,a   T. Kwaku Dayie*a  and  Herman O. Sintim*a  

* Corresponding authors

a Department of Chemistry and Biochemistry, University of Maryland, College Park, USA
E-mail: hsintim@umd.edu, dayie@umd.edu

Abstract

C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module.

Graphical abstract: Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy

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Article information

DOI
https://doi.org/10.1039/C2CC34379G
Article type
Communication
Submitted
19 Jun 2012
Accepted
19 Jul 2012
First published
20 Jul 2012

Chem. Commun., 2012,48, 9059-9061

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Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy

S. Nakayama, Y. Luo, J. Zhou, T. K. Dayie and H. O. Sintim, Chem. Commun., 2012, 48, 9059 DOI: 10.1039/C2CC34379G

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