Issue 71, 2012
From the journal:

Chemical Communications

Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases†‡

Amanda Beddows,§a   Nikesh Patel,§a   L. David Finger,a   John M. Atack,a   David M. Williamsa  and  Jane A. Grasby*a  

* Corresponding authors

a Centre for Chemical Biology, Department of Chemistry, Krebs Institute, University of Sheffield, Sheffield, UK
E-mail: j.a.grasby@sheffield.ac.uk
Tel: +44 (0)1142229478

Abstract

Flap endonucleases (FENs) are proposed to select their target phosphate diester by unpairing the two terminal nucleotides of duplex. Interstrand disulfide crosslinks, introduced by oxidation of thiouracil and thioguanine bases, abolished the specificity of human FEN1 for hydrolysis one nucleotide into the 5′-duplex.

Graphical abstract: Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases

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Article information

DOI
https://doi.org/10.1039/C2CC33400C
Article type
Communication
Submitted
10 May 2012
Accepted
10 Jul 2012
First published
11 Jul 2012

Chem. Commun., 2012,48, 8895-8897

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Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases

A. Beddows, N. Patel, L. D. Finger, J. M. Atack, D. M. Williams and J. A. Grasby, Chem. Commun., 2012, 48, 8895 DOI: 10.1039/C2CC33400C

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