Issue 48, 2012
From the journal:

Chemical Communications

Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale†

Steven P. Hanlon,a   Andrea Camattari,b   Sandra Abad,b   Anton Glieder,b   Matthias Kittelmann,c   Stephan Lütz,c   Beat Wirza  and  Margit Winkler*b  

* Corresponding authors

a F. Hoffmann-La Roche Ltd., 4070 Basel, Switzerland
Fax: +41 61 688 1673

b acib GmbH c/o Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, 8010 Graz, Austria
E-mail: margit.winkler@acib.at
Fax: +43 316 873 9302
Tel: +43 316 873 9333

c Novartis Pharma AG, 4070 Basel, Switzerland
Fax: +41 61 324 2103

Abstract

A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.

Graphical abstract: Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale

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Article information

DOI
https://doi.org/10.1039/C2CC17878H
Article type
Communication
Submitted
16 Dec 2011
Accepted
25 Apr 2012
First published
25 Apr 2012

Chem. Commun., 2012,48, 6001-6003

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Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale

S. P. Hanlon, A. Camattari, S. Abad, A. Glieder, M. Kittelmann, S. Lütz, B. Wirz and M. Winkler, Chem. Commun., 2012, 48, 6001 DOI: 10.1039/C2CC17878H

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