Jump to main content
Jump to site search

Issue 10, 2012
Previous Article Next Article

An optimized isotopic labelling strategy of isoleucine-γ2 methyl groups for solution NMR studies of high molecular weight proteins

Author affiliations

Abstract

An efficient synthetic route is proposed to produce 2-hydroxy-2-ethyl-3-oxobutanoate for the specific labelling of Ile methyl-γ2groups in proteins. The 2H, 13C-pattern of the biosynthetic precursor has been designed to optimize magnetization transfer, in large proteins, between these important structural probes and their corresponding backbone nuclei.

Graphical abstract: An optimized isotopic labelling strategy of isoleucine-γ2 methyl groups for solution NMR studies of high molecular weight proteins

Back to tab navigation

Supplementary files

Publication details

The article was received on 18 May 2011, accepted on 14 Jul 2011 and first published on 26 Jul 2011


Article type: Communication
DOI: 10.1039/C1CC12932E
Citation: Chem. Commun., 2012,48, 1434-1436
  •   Request permissions

    An optimized isotopic labelling strategy of isoleucine-γ2 methyl groups for solution NMR studies of high molecular weight proteins

    I. Ayala, O. Hamelin, C. Amero, O. Pessey, M. J. Plevin, P. Gans and J. Boisbouvier, Chem. Commun., 2012, 48, 1434
    DOI: 10.1039/C1CC12932E

Search articles by author

Spotlight

Advertisements