Preparation and characterization a new certified reference material of puerarin (GBW09511)

Abstract

Sample preparation, homogeneity study, stability study, value characterization, and evaluation of uncertainty of a new certified reference material (CRM) of puerarin are presented in this paper. The homogeneity was checked by a differential scanning calorimetry method. The stability was tested under different storage conditions. The CRM described above was homogeneous and stable at 25 °C for at least one year. The certified value of puerarin CRM is 99.66% with an extended uncertainty of 0.48% (k = 2). Coulometric titration and differential scanning calorimetry methods are used for purity determination of puerarin for the first time in this paper. The new CRM of puerarin can be used to validate analytical methods, improve the accuracy and comparability of measurement data, establish meteorological traceability of analytical results as well as control the quality of puerarin in relevant food and pharmaceutical formulations.