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Issue 12, 2012
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A novel approach for rapid determination of vitamin B12 in pharmaceutical preparations using BSA-modified gold nanoclusters

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Abstract

A simple, sensitive, and selective method based on the fluorescence quenching of BSA-stabilized gold nanoclusters (BSA-AuNCs) for rapid determination of vitamin B12 is developed. The fluorescence emission was measured at λex/λem 370/617 nm in phosphate buffer solution of pH 7.4, and the experimental variables and possible interference were studied. Under optimized conditions, the proposed method allows the determination of vitamin B12 over the range 160.0 ng mL−1 to 38.5 μg mL−1 with a correlation coefficient of 0.998 and a limit of quantification of 100.0 ng mL−1. The proposed method was successfully applied to commercial injection dosage forms. The recovery of the method is in the range of 97.7–102.0% and the relative standard deviation is in the range of 2.0%–5.9%.

Graphical abstract: A novel approach for rapid determination of vitamin B12 in pharmaceutical preparations using BSA-modified gold nanoclusters

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Publication details

The article was received on 24 Feb 2012, accepted on 02 Oct 2012 and first published on 02 Oct 2012


Article type: Paper
DOI: 10.1039/C2AY25196E
Citation: Anal. Methods, 2012,4, 4155-4160
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    A novel approach for rapid determination of vitamin B12 in pharmaceutical preparations using BSA-modified gold nanoclusters

    F. Samari, B. Hemmateenejad, Z. Rezaei and M. Shamsipur, Anal. Methods, 2012, 4, 4155
    DOI: 10.1039/C2AY25196E

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