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Issue 1, 2012
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Integrated DNA extraction and amplification using electrokinetic pumping in a microfluidic device

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Abstract

An integrated system employing anion exchange for the extraction of DNA from biological samples prior to polymerase chain reaction DNA amplification has been developed, based on microfluidic methodology utilising electrokinetic pumping. In this system, the biological samples were added directly to chitosan-coated silica beads to facilitate DNA immobilisation. The purified, pre-concentrated DNA was then eluted using a combination of electro-osmotic flow enhanced with electrophoretic mobility, which enable DNA to be transported by both mechanisms into the DNA amplification chamber. Through optimisation of the DNA elution conditions, average DNA extraction efficiencies of 69.1% were achievable. Subsequent DNA amplification performed on the microfluidic system demonstrated not only the ability to use electrokinetic movement to integrate the two processes on a single device, but also that the quality and quantity of DNA eluted was suitable for downstream analysis. This work offers an attractive real-world to chip interface and a route to simpler Lab-on-a-Chip technology which eliminates the need for moving parts.

Graphical abstract: Integrated DNA extraction and amplification using electrokinetic pumping in a microfluidic device

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Publication details

The article was received on 02 Sep 2011, accepted on 07 Oct 2011 and first published on 03 Nov 2011


Article type: Paper
DOI: 10.1039/C1AY05552F
Citation: Anal. Methods, 2012,4, 96-100
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    Integrated DNA extraction and amplification using electrokinetic pumping in a microfluidic device

    J. Parton, C. Birch, C. Kemp, S. J. Haswell, N. Pamme and K. J. Shaw, Anal. Methods, 2012, 4, 96
    DOI: 10.1039/C1AY05552F

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