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Issue 17, 2012
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13C cell wall enrichment and ionic liquid NMR analysis: progress towards a high-throughput detailed chemical analysis of the whole plant cell wall

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Abstract

The ability to accurately and rapidly measure plant cell wall composition, relative monolignol content and lignin–hemicellulose inter-unit linkage distributions has become essential to efforts centered on reducing the recalcitrance of biomass by genetic engineering. Growing 13C enriched transgenic plants is a viable route to achieve the high-throughput, detailed chemical analysis of whole plant cell wall before and after pretreatment and microbial or enzymatic utilization by 13C nuclear magnetic resonance (NMR) in a perdeuterated ionic liquid solvent system not requiring component isolation. 1D 13C whole cell wall ionic liquid NMR of natural abundant and 13C enriched corn stover stem samples suggest that a high level of uniform labeling (>97%) can significantly reduce the total NMR experiment times up to ∼220 times. Similarly, significant reduction in total NMR experiment time (∼39 times) of the 13C enriched corn stover stem samples for 2D 13C–1H heteronuclear single quantum coherence NMR was found.

Graphical abstract: 13C cell wall enrichment and ionic liquid NMR analysis: progress towards a high-throughput detailed chemical analysis of the whole plant cell wall

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Publication details

The article was received on 13 Mar 2012, accepted on 29 May 2012 and first published on 05 Jul 2012


Article type: Communication
DOI: 10.1039/C2AN35344J
Citation: Analyst, 2012,137, 3904-3909
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    13C cell wall enrichment and ionic liquid NMR analysis: progress towards a high-throughput detailed chemical analysis of the whole plant cell wall

    M. Foston, R. Samuel and A. J. Ragauskas, Analyst, 2012, 137, 3904
    DOI: 10.1039/C2AN35344J

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