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Issue 13, 2012
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Simplified sheath flow cuvette design for ultrasensitive laser induced fluorescence detection in capillary electrophoresis

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Abstract

We present a design for a sheath-flow cuvette that uses a relatively inexpensive quartz cuvette. The cuvette has a high optical quality square flow chamber that is fused to quartz tubes at each end. PEEK/TEFZEL fittings hold and seal the quartz flow chamber without putting strain on the cuvette. The performance of the cuvette is evaluated as a laser-induced fluorescence detector for capillary electrophoresis. The cuvette produces mass detection limits of 50 yoctomoles (30 copies) for 5-carboxyl tetramethylrhodamine (5 TAMRA SE) with a separation efficiency of 400 000 theoretical plates.

Graphical abstract: Simplified sheath flow cuvette design for ultrasensitive laser induced fluorescence detection in capillary electrophoresis

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Publication details

The article was received on 06 Mar 2012, accepted on 11 Apr 2012, published on 13 Apr 2012 and first published online on 13 Apr 2012


Article type: Paper
DOI: 10.1039/C2AN35321K
Citation: Analyst, 2012,137, 3099-3101
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    Simplified sheath flow cuvette design for ultrasensitive laser induced fluorescence detection in capillary electrophoresis

    O. O. Dada, B. J. Huge and N. J. Dovichi, Analyst, 2012, 137, 3099
    DOI: 10.1039/C2AN35321K

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