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Issue 13, 2012
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Sampling techniques for single-cell electrophoresis

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Abstract

Cells are extraordinarily complex, containing thousands of different analytes with concentrations spanning at least nine orders of magnitude. Analyzing single cells instead of tissue homogenates provides unique insights into cell-to-cell heterogeneity and aids in distinguishing normal cells from pathological ones. The high sensitivity and low sample consumption of capillary and on-chip electrophoresis, when integrated with fluorescence, electrochemical, and mass spectrometric detection methods, offer an ideal toolset for examining single cells and even subcellular organelles; however, the isolation and loading of such small samples into these devices is challenging. Recent advances have addressed this issue by interfacing a variety of enhanced mechanical, microfluidic, and optical sampling techniques to capillary and on-chip electrophoresis instruments for single-cell analyses.

Graphical abstract: Sampling techniques for single-cell electrophoresis

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Publication details

The article was received on 05 Dec 2011, accepted on 15 Jan 2012 and first published on 30 Jan 2012


Article type: Minireview
DOI: 10.1039/C2AN16211C
Citation: Analyst, 2012,137, 2922-2929
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    Sampling techniques for single-cell electrophoresis

    C. Cecala and J. V. Sweedler, Analyst, 2012, 137, 2922
    DOI: 10.1039/C2AN16211C

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