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Issue 4, 2012
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Selective enrichment and sensitive detection of peptide and protein biomarkers in human serum using polymeric reverse micelles and MALDI-MS

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Abstract

Reverse-micelle forming amphiphilic homopolymers with carboxylic acid and quaternary amine substituents are used to selectively enrich biomarker peptides and protein fragments from human serum prior to matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. After depletion of human serum albumin (HSA) and immunoglobulin G (IgG), low abundance peptide biomarkers can be selectively enriched and detected by MALDI-MS at clinically relevant concentrations by using the appropriate homopolymer(s) and extraction pH value(s). Three breast cancer peptide biomarkers, bradykinin, C4a, and ITIH4, were chosen to test this new approach, and detection limits of 0.5 ng mL−1, 0.08 ng mL−1, and 0.2 ng mL−1, respectively, were obtained. In addition, the amphiphilic homopolymers were used to detect prostate specific antigen (PSA) at concentrations as low as 0.5 ng mL−1 by targeting a surrogate peptide fragment of this protein biomarker. Selective enrichment and sensitive MS detection of low abundance peptide/protein biomarkers by these polymeric reverse micelles should be a sensitive and straightforward approach for biomarker screening in human serum.

Graphical abstract: Selective enrichment and sensitive detection of peptide and protein biomarkers in human serum using polymeric reverse micelles and MALDI-MS

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Publication details

The article was received on 10 Nov 2011, accepted on 08 Dec 2011 and first published on 23 Dec 2011


Article type: Paper
DOI: 10.1039/C2AN16089G
Citation: Analyst, 2012,137, 1024-1030
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    Selective enrichment and sensitive detection of peptide and protein biomarkers in human serum using polymeric reverse micelles and MALDI-MS

    N. Rodthongkum, R. Ramireddy, S. Thayumanavan and W. V. Richard, Analyst, 2012, 137, 1024
    DOI: 10.1039/C2AN16089G

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