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Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Science, 7–45 Songdo-dong, Yeonsu-ku, South Korea
E-mail: hklee@gachon.ac.kr
; Fax: +82 32 820 6519
; Tel: +82 32 820 6584
b
Department of Chemistry, Yonsei University, Seoul, South Korea
E-mail: dongil@yonsei.ac.kr
; Fax: +822 3647050
; Tel: +822 21235638
Analyst, 2012,137, 991-998
DOI:
10.1039/C1AN15810D
Received
02 Sep 2011,
Accepted
18 Nov 2011
First published online
21 Dec 2011
In recent years, gold nanoparticles have been increasingly utilized as a promising material for biomedical analysis. We report here for the first time the synthesis of ultrasmall gold nanoparticles with core diameter of 1.2 nm functionalized with hydrazidegroups and their use in isolation/enrichment of N-glycosylated peptides. Hydrazide-functionalized gold nanoparticles showed excellent stability in biological samples and exhibited a large capacity for peptide capturing. The captured peptides from tested standard glycoproteins were found to be highly specific as determined by Agilent HPLC chip and quadrupole time-of-flight (Q-TOF) mass spectrometer. The hydrazide-functionalized gold nanoparticles were successfully utilized in the isolation of a real proteome complex, which showed that more than 90% of captured product was glycopeptide. These results demonstrate that the ultrasmall gold nanoparticles can be used for a high-throughput analysis platform of glycoproteins.
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