Jump to main content
Jump to site search

Issue 1, 2013
Previous Article Next Article

The muraminomicin biosynthetic gene cluster and enzymatic formation of the 2-deoxyaminoribosyl appendage

Author affiliations

Abstract

Muraminomicin is a lipopeptidyl nucleoside antibiotic produced by Streptosporangium amethystogenes SANK 60709. Similar to several members of this antibiotic family such as A-90289 and muraymycin, the structure of muraminomicin consists of a disaccharide comprised of two modified ribofuranose units linked by an O-β(1 → 5) glycosidic bond; however, muraminomicin holds the distinction in that both ribose units are 2-deoxy sugars. The biosynthetic gene cluster of muraminomicin has been identified, cloned and sequenced, and bioinformatic analysis revealed a minimum of 24 open reading frames putatively involved in the biosynthesis, resistance, and regulation of muraminomicin. Fives enzymes are likely involved in the assembly and attachment of the 2,5-dideoxy-5-aminoribose saccharide unit, and two are now functionally assigned and characterized: Mra20, a 5′-amino-2′,5′-dideoxyuridine phosphorylase and Mra23, a UTP:5-amino-2,5-dideoxy-α-D-ribose-1-phosphate uridylyltransferase. The cumulative results are consistent with the incorporation of the ribosyl appendage of muraminomicin via the archetypical sugar biosynthetic pathway that parallels A-90289 biosynthesis, and the specificity for this appendage is dictated primarily by the two characterized enzymes.

Graphical abstract: The muraminomicin biosynthetic gene cluster and enzymatic formation of the 2-deoxyaminoribosyl appendage

  • This article is part of the themed collection: New Talent
Back to tab navigation

Supplementary files

Publication details

The article was received on 17 Aug 2012, accepted on 18 Sep 2012 and first published on 26 Sep 2012


Article type: Concise Article
DOI: 10.1039/C2MD20245J
Citation: Med. Chem. Commun., 2013,4, 239-243
  •   Request permissions

    The muraminomicin biosynthetic gene cluster and enzymatic formation of the 2-deoxyaminoribosyl appendage

    X. Chi, S. Baba, N. Tibrewal, M. Funabashi, K. Nonaka and S. G. Van Lanen, Med. Chem. Commun., 2013, 4, 239
    DOI: 10.1039/C2MD20245J

Search articles by author

Spotlight

Advertisements